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KMID : 0351219950270040397
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Korean Journal of Infectious Diseases
1995 Volume.27 No. 4 p.397 ~ p.402
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Comparison of Enzyme Immunoassay and Latex Agglutination for Direct Detection of streptococcus pyogenes from the Throat
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Abstract
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Background:
@EN Although throat culture is a golden standard to diagnose bacterial pharyngitis, it takes about two days to isolate and identify Streptococcus pyogenes. The author evaluated two commercial kits for direct detection of S. pyogenes from the
throat
which can save time in throat culture.
@ES Methods:
@EN Throat culture was taken from One-hundred ninty-nine school children and direct antigen tests for S. pyogenes using TestPack Strep A (Abbott. USA) based on enzyme immunoassay and A Strep AD (Denka Seiken, Japan) based on latex agglutination
were
done.
@ES Results:
@EN Fifty-nine children (29.7%) were positive for S. pyogenes. TestPack Strep A showed a better sensitivity of 61% and a better specificity of 100% compared with those of 18.9%, 99.2% of A Strep AD, respectively. The result of TestPack Strep A
showed a
better correlation with the number of colonies of S. pyogenes (r=0.39, P <0.01) than A Strep AD (r=0.04).
@ES Conclusion:
@EN The sensitivity of A Strep AD shown is too low to be used in the diagnosis of streptococal pharyngitis and that of TestPack Strep A is not sufficiently high to rule out streptococcal pharyngitis for the negative result.
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KEYWORD
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